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KMID : 0380020180330030168
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Korean Journal of Biotechnology and Bioengineering
2018 Volume.33 No. 3 p.168 ~ p.174
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Bioaccumulation of Copper-L-Lysine Chelate in Bacillus megaterium
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Park Jae-Ho
Park Hye-Min
Yun Hyun-Shik
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Abstract
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Copper is a bivalent mineral and nutritionally necessary for mammals. It has been used as an animal feed additive or growth factor for animals. However, large amount of copper contained in animal manure by oversupplying of copper have a negative impact on the soil environment. Chelates of copper and amino acids have the advantages of being better absorbed into the intestines than copper ions and less sensitive to photodegradation than amino acids. Bacillus spp., a spore-forming probiotic, have stability against heat and low pH and are used as animal growth factor or feed additives. In this study, copper-L-lysine chelate was accumulated in Bacillus megaterium ATCC 10778 to use as mineral-containing probiotics or feed additives. The highest chelation rate of copper sulfate and L-lysine was 62.53% after 1:1 reaction of copper sulfate to L-lysine for 25 minutes at 70oC. FB media was selected for bioaccumulation of copper-L-lysine chelate in flask culture. In the fermentor culture, 38,043 ppm of copper ion was accumulated in 6.50×108 CFU/mL of B. megaterium.
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KEYWORD
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copper, chelate, L-Lysine, Bacillus megaterium, bioaccumulation
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